Recombinant cold-adapted attenuated influenza A vaccines for use in children: molecular genetic analysis of the cold-adapted donor and recombinants.
Identifieur interne : 002489 ( Main/Exploration ); précédent : 002488; suivant : 002490Recombinant cold-adapted attenuated influenza A vaccines for use in children: molecular genetic analysis of the cold-adapted donor and recombinants.
Auteurs : Y Z Ghendon ; F I Polezhaev ; K V Lisovskaya ; T E Medvedeva ; G I Alexandrova ; A I KlimovSource :
- Infection and immunity [ 0019-9567 ] ; 1984.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ADN recombiné, ADN viral.
- génétique : Hémagglutinines virales, Sialidase, Virus de la grippe A.
- Animaux, Basse température, Embryon de poulet, Phénotype, Vaccins antigrippaux, Vaccins atténués.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : DNA, Recombinant, DNA, Viral.
- chemical , genetics : Hemagglutinins, Viral, Neuraminidase.
- genetics : Influenza A virus.
- Animals, Chick Embryo, Cold Temperature, Influenza Vaccines, Phenotype, Vaccines, Attenuated.
Abstract
A previously described cold-adapted attenuated virus, A/Leningrad/134/17/57 (H2N2), was further modified by 30 additional passages in chicken embryos at 25 degrees C. This virus had a distinct temperature-sensitive (ts) phenotype, grew well in chicken embryos at 25 degrees C, and failed to recombine with reference ts mutants of fowl plague virus containing ts lesions in five genes coding for non-glycosylated proteins (genes 1, 2, 5, 7, and 8). Recombination of A/Leningrad/134/47/57 with wild-type influenza virus strains A/Leningrad/322/79 (H1N1) and A/Bangkok/1/79(H3N2) yielded ts recombinants 47/25/1(H1N1) and 47/7/2 (H3N2). These recombinants inherited their ts phenotype and ability to reproduce in chicken embryos at 25 degrees C from the cold-adapted parent. Analysis of the genome composition of the recombinants obtained by recombination of the cold-adapted donor with wild-type influenza virus strains A/Leningrad/322/79(H1N1) and A/Bangkok/1/79(H3N2) showed that recombinants 47/25/1(H1N1) and 47/7/2 (H3N2) inherited five and six genes, respectively, from the cold-adapted parent, and hemagglutinin and neuraminidase genes from the wild-type strains.
PubMed: 6724694
Affiliations:
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uniqKey="Klimov A" first="A I" last="Klimov">A I Klimov</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1984">1984</date><idno type="RBID">pubmed:6724694</idno><idno type="pmid">6724694</idno><idno type="wicri:Area/PubMed/Corpus">000521</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000521</idno><idno type="wicri:Area/PubMed/Curation">000521</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000521</idno><idno type="wicri:Area/PubMed/Checkpoint">000460</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000460</idno><idno type="wicri:Area/Ncbi/Merge">001217</idno><idno type="wicri:Area/Ncbi/Curation">001217</idno><idno type="wicri:Area/Ncbi/Checkpoint">001217</idno><idno type="wicri:doubleKey">0019-9567:1984:Ghendon Y:recombinant:cold:adapted</idno><idno type="wicri:Area/Main/Merge">002613</idno><idno type="wicri:Area/Main/Curation">002489</idno><idno type="wicri:Area/Main/Exploration">002489</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Recombinant cold-adapted attenuated influenza A vaccines for use in children: molecular genetic analysis of the cold-adapted donor and recombinants.</title><author><name sortKey="Ghendon, Y Z" sort="Ghendon, Y Z" uniqKey="Ghendon Y" first="Y Z" last="Ghendon">Y Z Ghendon</name></author><author><name sortKey="Polezhaev, F I" sort="Polezhaev, F I" uniqKey="Polezhaev F" first="F I" last="Polezhaev">F I Polezhaev</name></author><author><name sortKey="Lisovskaya, K V" sort="Lisovskaya, K V" uniqKey="Lisovskaya K" first="K V" last="Lisovskaya">K V Lisovskaya</name></author><author><name sortKey="Medvedeva, T E" sort="Medvedeva, T E" uniqKey="Medvedeva T" first="T E" last="Medvedeva">T E Medvedeva</name></author><author><name sortKey="Alexandrova, G I" sort="Alexandrova, G I" uniqKey="Alexandrova G" first="G I" last="Alexandrova">G I Alexandrova</name></author><author><name sortKey="Klimov, A I" sort="Klimov, A I" uniqKey="Klimov A" first="A I" last="Klimov">A I Klimov</name></author></analytic><series><title level="j">Infection and immunity</title><idno type="ISSN">0019-9567</idno><imprint><date when="1984" type="published">1984</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chick Embryo</term><term>Cold Temperature</term><term>DNA, Recombinant (analysis)</term><term>DNA, Viral (analysis)</term><term>Hemagglutinins, Viral (genetics)</term><term>Influenza A virus (genetics)</term><term>Influenza Vaccines</term><term>Neuraminidase (genetics)</term><term>Phenotype</term><term>Vaccines, Attenuated</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN recombiné (analyse)</term><term>ADN viral (analyse)</term><term>Animaux</term><term>Basse température</term><term>Embryon de poulet</term><term>Hémagglutinines virales (génétique)</term><term>Phénotype</term><term>Sialidase (génétique)</term><term>Vaccins antigrippaux</term><term>Vaccins atténués</term><term>Virus de la grippe A (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Recombinant</term><term>DNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Hemagglutinins, Viral</term><term>Neuraminidase</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN recombiné</term><term>ADN viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Hémagglutinines virales</term><term>Sialidase</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chick Embryo</term><term>Cold Temperature</term><term>Influenza Vaccines</term><term>Phenotype</term><term>Vaccines, Attenuated</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Basse température</term><term>Embryon de poulet</term><term>Phénotype</term><term>Vaccins antigrippaux</term><term>Vaccins atténués</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A previously described cold-adapted attenuated virus, A/Leningrad/134/17/57 (H2N2), was further modified by 30 additional passages in chicken embryos at 25 degrees C. This virus had a distinct temperature-sensitive (ts) phenotype, grew well in chicken embryos at 25 degrees C, and failed to recombine with reference ts mutants of fowl plague virus containing ts lesions in five genes coding for non-glycosylated proteins (genes 1, 2, 5, 7, and 8). Recombination of A/Leningrad/134/47/57 with wild-type influenza virus strains A/Leningrad/322/79 (H1N1) and A/Bangkok/1/79(H3N2) yielded ts recombinants 47/25/1(H1N1) and 47/7/2 (H3N2). These recombinants inherited their ts phenotype and ability to reproduce in chicken embryos at 25 degrees C from the cold-adapted parent. Analysis of the genome composition of the recombinants obtained by recombination of the cold-adapted donor with wild-type influenza virus strains A/Leningrad/322/79(H1N1) and A/Bangkok/1/79(H3N2) showed that recombinants 47/25/1(H1N1) and 47/7/2 (H3N2) inherited five and six genes, respectively, from the cold-adapted parent, and hemagglutinin and neuraminidase genes from the wild-type strains.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Alexandrova, G I" sort="Alexandrova, G I" uniqKey="Alexandrova G" first="G I" last="Alexandrova">G I Alexandrova</name><name sortKey="Ghendon, Y Z" sort="Ghendon, Y Z" uniqKey="Ghendon Y" first="Y Z" last="Ghendon">Y Z Ghendon</name><name sortKey="Klimov, A I" sort="Klimov, A I" uniqKey="Klimov A" first="A I" last="Klimov">A I Klimov</name><name sortKey="Lisovskaya, K V" sort="Lisovskaya, K V" uniqKey="Lisovskaya K" first="K V" last="Lisovskaya">K V Lisovskaya</name><name sortKey="Medvedeva, T E" sort="Medvedeva, T E" uniqKey="Medvedeva T" first="T E" last="Medvedeva">T E Medvedeva</name><name sortKey="Polezhaev, F I" sort="Polezhaev, F I" uniqKey="Polezhaev F" first="F I" last="Polezhaev">F I Polezhaev</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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